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	Author	Lacan Marie, Thèves Catherine, Keyser Christine, Farrugia Audrey, Baraybar Jose-Pablo, Crubézy Eric, Ludes Bertrand,
	Title	Detection of age-related duplications in mtDNA from human muscles and bones.			Type	Journal Article
	Year	2011	Publication	International journal of legal medicine	Abbreviated Journal
	Volume	125	Issue	2	Pages	293 -300
	Keywords	Adolescent, Adult, Aged, Aged, 80 and over, Aging, Aging: genetics, Child, Preschool, DNA, Mitochondrial, DNA, Mitochondrial: genetics, Female, Humans, Intercostal Muscles, Intercostal Muscles: metabolism, Male, Middle Aged, Point Mutation, Ribs, Ribs: metabolism, Young Adult,
	Abstract	Several studies have demonstrated the age-related accumulation of duplications in the D-loop of mitochondrial DNA (mtDNA) extracted from skeletal muscle. This kind of mutation had not yet been studied in bone. The detection of age-related mutations in bone tissue could help to estimate age at death within the context of legal medicine or/and anthropological identification procedures, when traditional osteological markers studied are absent or inefficient. As we detected an accumulation of a point mutation in mtDNA from an older individual's bones in a previous study, we tried here to identify if three reported duplications (150, 190, 260 bp) accumulate in this type of tissue. We developed a sensitive method which consists in the use of back-to-back primers during amplification followed by an electrophoresis capillary analysis. The aim of this study was to confirm that at least one duplication appears systematically in muscle tissue after the age of 20 and to evaluate the duplication age appearance in bones extracted from the same individuals. We found that the number of duplications increase from 38 years and that at least one duplicated fragment is present in 50% of cases after 70 years in this tissue. These results confirm that several age-related mutations can be detected in the D-loop of mtDNA and open the way for the use of molecular markers for age estimation in forensic and/or anthropological identification.
	Programme	1038
	Campaign
	Address
	Corporate Author				Thesis
	Publisher		Place of Publication		Editor
	Language		Summary Language		Original Title
	Series Editor		Series Title		Abbreviated Series Title
	Series Volume		Series Issue		Edition
	ISSN	1340-9050	ISBN		Medium
	Area		Expedition		Conference
	Notes				Approved	yes
	Call Number				Serial	2692
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