Abstract: Within ecophysiological and genetic studies on insects, morphological and physiological traits are commonly assessed and phenotypes are typically obtained from manual measurements on numerous individuals. Manual observations are, however, time consuming, can introduce observer bias and are prone to human error. Here, we contrast results obtained from manual assessment of larval size and thermal tolerance traits in black soldier flies (Hermetia illucens) and houseflies (Musca domestica) that have been acclimated under three different temperature regimes with those obtained automatically using an image analysis software (Noldus EthoVision XT). We found that (i) larval size estimates of both species, obtained by manual weighing or by using the software, were highly correlated, (ii) measures of heat and cold tolerance using manual and automated approaches provided qualitatively similar results, and (iii) by using the software we obtained quantifiable information on stress responses and acclimation effects of potentially higher ecological relevance than the endpoint traits that are typically assessed when manual assessments are used. Based on these findings, we argue that automated assessment of insect stress responses and largescale phenotyping of morphological traits such as size will provide new opportunities within many disciplines where accurate and largescale phenotyping of insects is required.

Abstract: Climate And Water Cycle Reconstruction Over The Last Centuries And Prediction Should Be Improved In The Southern Hemisphere (E.g. Last Ipcc Report). First, Direct Observations Are Scarce (E.g. In Antarctica, Mainly Basic Automatic Weather Station – Aws – Only Since The 50’s And Satellite Observations). As A Consequence, Climate And Water Cycle Reconstructions Are Largely Dependent On Proxy Measurements Such As Water Isotopes In Polar Ice Cores, Even Over The Last Century. Second, Climate And Water Cycle Reconstructions Using Models Should Be Improved. In Particular, Problems Have Been Identified For (1) Estimation Of Precipitation Amounts (Large Discrepancies Among Models Over The Austral Ocean And The Ice-sheet), (2) Description Of Particular Atmospheric Processes Associated With Cloud Microphysics And Exchanges Between Surface And Atmosphere In Polar Regions And (3) Seasonal To Interannual Evolution Of The Locations Of The Westerlies And Subtropical Jet And Associated Air Mass Trajectories. The Last Aspect Has Important Direct Consequences For The Atmospheric Dynamics In This Region But Also Indirect Consequences Such As The Links Between Westerlies, Co2 Outgassing And Sea Ice Extent In The Austral Ocean (Saunders Et Al. 2018; Menviel Et Al. 2018; Holland And Kwok, 2012). With The Objectives To Provide (1) A Better Description Of Water Cycle Dynamic In The Southern Ocean At Annual And Interannual Scales And (2) An Improved Transfer Function Between Climate / Water Cycle And Water Isotopes In This Region, A Bunch Of Instruments Measuring Continuously The Isotopic Composition Of Water In The Water Vapor And In The Precipitation Has Been Installed Over The Last Years Along A Transect From La Réunion To Antarctica.first Results From The Antarctic Instruments Reveal Clear Isotopic Signatures Of Large Scale Water Cycle Features Such As Atmospheric Rivers Dominating The Surface Mass Balance In Antarctica. In La Réunion, An Extensive Study Of The Drivers Of The Isotopic Composition Of The Water Vapor Showed That The Influence Of The Subtropical Westerly Jet Dominates The Water Isotopic Signal At Night. To Connect The Two Regions, An Instrument Is Running On Amsterdam Island Since November 2019 With First Results Showing A Clear Isotopic Signature During The High Pressure And Low Pressure Synoptic Events. Still, No In-depth Study Of The Data Trying To Connect The Isotopic Record In Amsterdam Island To Larger Scale Patterns Of The Water Cycle Has Been Initiated.the Goal Of This Internship Is To Provide The First Analyses Of The Isotopic (Water Vapor And Precipitation) Records On Amsterdam Island With A Comparison With Meteorological Data And Environmental Data Collected In Parallel On The Observatory Of Amsterdam Island (E.g. Gaseous Elemental Mercury). After These First Analyses Of The Data, Comparisons Will Be Performed With Water Isotopic Records In La Réunion Over Selected Events (Subtropical Jet Or Cold Fronts Intrusions). This Study Will Involve Analyses Of Meteorological Maps As Well As Backtrajectories.this Internship Requires Skills For (Python Or R Languages) And Involves Large Interactions Within A Large Research Team.

Abstract: The eradication of invasive mammals on islands is important for protecting seabird populations and insular ecosystems. However, the impacts of such eradications are insufficiently known because monitoring of potentially beneficiary species is often sporadic and limited. We performed a survey of all seabird species on Saint-Paul Island, southern Indian Ocean, 20 years after successful eradication of invasive black rat (Rattus rattus) and European rabbit (Oryctolagus cuniculus). Using complementary sampling designs including adaptive cluster sampling, stratified random sampling and entire sampling, we estimated population changes and colonization by new seabird species. A total of 13 seabird species were found breeding on Saint-Paul post-eradication compared to six before the eradication. Among the seven species that colonized the island, five (MacGillivray’s prion Pachyptila macgillivrayi, fairy prion P. turtur, white-bellied storm petrel Fregetta grallaria, Antarctic tern Sterna vittata, sooty tern Onychoprion fuscatus) had relictual populations breeding on a nearby islet, and one (brown skua Catharacta antarctica) was a new breeding species. We also found breeding subantarctic little shearwaters Puffinus elegans. For species that were breeding on the Saint Paul pre-eradication, the mean annual population growth rate was 1.030 ± 0.093 (SE). Species known to be vulnerable to rat predation (prions, great-winged petrel Pterodroma macroptera, flesh-footed shearwater Puffinus carneipes, subantarctic little shearwater, white-bellied storm petrel, Antarctic tern) had the highest population growth rates. Two decades after the eradication of invasive mammals on a remote oceanic island, seabird populations were high beneficiaries. These findings further highlight the importance of invasive mammal eradication on islands as a conservation tool. Results are encouraging for the planned eradication of invasive mammals from nearby Amsterdam Island, and suggest this will mainly benefit terns and small burrowing petrels.

Abstract: Sexual competition is increasingly recognized as an important selective pressure driving species distributions. However, few studies have investigated the relative importance of interpopulation versus intrapopulation competition in relation to habitat availability and selection. To explain spatial segregation between sexes that often occurs in non-territorial and central place foragers, such as seabirds, two hypotheses are commonly used. The ‘competitive exclusion’ hypothesis states that dominant individuals should exclude subordinate individuals through direct competition, whereas the ‘niche divergence’ hypothesis states that segregation occurs due to past competition and habitat specialization. We tested these hypotheses in two populations of an extreme wide-ranging and sexually dimorphic seabird, investigating the relative role of intrapopulation and interpopulation competition in influencing sex-specific distribution and habitat preferences. Using GPS loggers, we tracked 192 wandering albatrosses Diomedea exulans during four consecutive years (2016–2019), from two neighbouring populations in the Southern Ocean (Prince Edward and Crozet archipelagos). We simulated pseudo-tracks to create a null spatial distribution and used Kernel Density Estimates (KDE) and Resource Selection Functions (RSF) to distinguish the relative importance of within- versus between-population competition. Kernel Density Estimates showed that only intrapopulation sexual segregation was significant for each monitoring year, and that tracks between the two colonies resulted in greater overlap than expected from the null distribution, especially for the females. RSF confirmed these results and highlighted key at-sea foraging areas, even if the estimated of at-sea densities were extremely low. These differences in selected areas between sites and sexes were, however, associated with high interannual variability in habitat preferences, with no clear specific preferences per site and sex. Our results suggest that even with low at-sea population densities, historic intrapopulation competition in wide-ranging seabirds may have led to sexual dimorphism and niche specialization, favouring the ‘niche divergence’ hypothesis. In this study, we provide a protocol to study competition within as well as between populations of central place foragers. This is relevant for understanding their distribution patterns and population regulation, which could potentially improve management of threatened populations.