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Agabi, A.; Aristidi, E.; Azouit, M.; Fossat, E.; Martin, F.; Sadibekova, T.; Vernin, J.; Ziad, A. (2006). First Whole Atmosphere Nighttime Seeing Measurements at Dome C, Antarctica. Publ. Astron. Soc. Pac., 118(840), 344–348.
Abstract: We report site-testing results obtained in the nighttime during the polar autumn and winter at Dome C. These results were collected during the first Concordia winterover by A. Agabi. They are based on seeing and isoplanatic angle monitoring, as well as in situ balloon measurements of the refractive index structure constant profiles C2n(h). Atmosphere is divided into two regions: (1) a 36 m high surface layer responsible for 87% of the turbulence, and (2) a very stable free atmosphere above, with a median seeing of 0.36“ +/- 0.19” at an elevation of h=30 m. The median seeing measured with a differential image motion monitor placed on top of an 8.5 m high tower is 1.3“ +/- 0.8”.
Keywords: Site Testing
Programme: 908
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Degletagne Cyril, Keime Celine, Rey Benjamin, de Dinechin Marc, Forcheron Fabien, Chuchana Paul, Jouventin Pierre, Gautier Christian, Duchamp Claude,. (2010). Transcriptome analysis in non-model species: a new method for the analysis of heterologous hybridization on microarrays. BMC Genomics, 11(1), 344.
Abstract: BACKGROUND:Recent developments in high-throughput methods of analyzing transcriptomic profiles are promising for many areas of biology, including ecophysiology. However, although commercial microarrays are available for most common laboratory models, transcriptome analysis in non-traditional model species still remains a challenge. Indeed, the signal resulting from heterologous hybridization is low and difficult to interpret because of the weak complementarity between probe and target sequences, especially when no microarray dedicated to a genetically close species is available.RESULTS:We show here that transcriptome analysis in a species genetically distant from laboratory models is made possible by using MAXRS, a new method of analyzing heterologous hybridization on microarrays. This method takes advantage of the design of several commercial microarrays, with different probes targeting the same transcript. To illustrate and test this method, we analyzed the transcriptome of king penguin pectoralis muscle hybridized to Affymetrix chicken microarrays, two organisms separated by an evolutionary distance of approximately 100 million years. The differential gene expression observed between different physiological situations computed by MAXRS was confirmed by real-time PCR on 10 genes out of 11 tested.CONCLUSIONS:MAXRS appears to be an appropriate method for gene expression analysis under heterologous hybridization conditions.
Programme: 131
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Razouls S., Razouls C. & Debovee F. (2000). Biodiversity and biogeography of Antarctic copepods. Antarct. Sci., 12(3), 343–362.
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André M.F. & Mercier D. (2003). Recherches récentes en milieux polaires et subpolaires. Bulletin de l'association des géographes français, 4, 343–416.
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Bailleul F., Charrassin J.-B., Ezraty R., Girard-Ardhuin F., McMahon C.R., Field I.C. & Guinet C. (2007). Southern elephant seals from Kerguelen Islands confronted by Antarctic sea ice. Changes in movements and in diving behaviour. Deep Sea Res. Part II Top. Stud. Oceanogr., 54, 343–355.
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Bailleul F., Charrassin J.B., Ezraty R., Girard-Ardhuin F., McMahon C.R., Field I.C, Guinet C. (2007). Southern Ocean investigated by elephant seals from Kerguelen Islands: movements and diving behaviour related to areas prospected. Deep Sea Res. Part II Top. Stud. Oceanogr., 54, 343–355.
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Mäkinen J., Amalvict M., Shibuya K. & Fukuda Y. (2007). Absolute Gravimetry in Antarctica: State-of-the-Art and Prospective. Journal of geodynamics, 43, 339–357.
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Razouls C. (1992). Inventaire des copépodes planctoniques marins antarctiques et subantarctiques. Vie et milieu, 42(3-4), 337–343.
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Raynaud D, V Lipenkov, B Lemieux-Dudon, P Duval, M-F Loutre, N Lhomme. (2007). The local insolation signature of air content in Antarcticice. A new step toward an absolute dating of ice records. Earth Planet. Sci. Lett., 261, 337–349.
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Ancel A., Kooyman G.L., Ponganis P.J., Gendner J.P., Lignon J., Mestre X., Huin N., Thorson P.H., Robisson P. & Le Maho Y. (1992). Foraging behaviour of emperor penguins as a resource detector in winter and summer. Nature, 360, 336–338.
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